The UNI Museum contains a wide variety of rare and unusual exhibits, including items of historical, geological and anthropological interest. Historical items include collections of weapons, coins, utensils, clothing, and agricultural implements. The collection of pre-Columbian artifacts is extensive, and includes objects from both Americas. Items of special interest, such as a locally excavated mastodon tusk and items pertaining to the history of the University, are also housed here.

The UNI museum was established in the 1890s and is used for educational purposes by a number of UNI departments. It has a full-time curator and is accredited by the American Association of Museums.